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cas9 ng gene  (Integrated DNA Technologies)


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    Structured Review

    Integrated DNA Technologies cas9 ng gene
    ( A ) Chromosomal construction of Escherichia coli HK1160 ( P BAD -dCas9-NG-KmR) and structure of sgRNA plasmid. dCas9-NG protein is expressed by L-arabinose-inducible P BAD promoter. sgRNA is constitutively expressed by P j23119 promoter. ( B ) Regulation of gal operon transcription by dCas9-NG and sgRNA complex. In the absence of L-arabinose, dCas9-NG is not expressed and gal transcription can be initiated by RNA polymerase. In the presence of L-arabinose, dCas9-NG is expressed and dCas9-NG-sgRNA complex recognizes target DNA adjacent to functional PAM sequence. ( C ) Switch on/off of L-arabinose-inducible CRISPRi. In the presence of L-arabinose, <t>Cas9</t> and dCas9 are expressed to repress the expression of D-galactose metabolizing enzymes (white colonies on MacConkey agar containing D-galactose). In the absence of L-arabinose, cells can utilize D-galactose (red colonies).
    Cas9 Ng Gene, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 4157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+ng+gene/pmc09728369-69-5-14?v=Integrated+DNA+Technologies
    Average 99 stars, based on 4157 article reviews
    cas9 ng gene - by Bioz Stars, 2026-07
    99/100 stars

    Images

    1) Product Images from "Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference"

    Article Title: Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference

    Journal: Journal of Microbiology and Biotechnology

    doi: 10.4014/jmb.2008.08058

    ( A ) Chromosomal construction of Escherichia coli HK1160 ( P BAD -dCas9-NG-KmR) and structure of sgRNA plasmid. dCas9-NG protein is expressed by L-arabinose-inducible P BAD promoter. sgRNA is constitutively expressed by P j23119 promoter. ( B ) Regulation of gal operon transcription by dCas9-NG and sgRNA complex. In the absence of L-arabinose, dCas9-NG is not expressed and gal transcription can be initiated by RNA polymerase. In the presence of L-arabinose, dCas9-NG is expressed and dCas9-NG-sgRNA complex recognizes target DNA adjacent to functional PAM sequence. ( C ) Switch on/off of L-arabinose-inducible CRISPRi. In the presence of L-arabinose, Cas9 and dCas9 are expressed to repress the expression of D-galactose metabolizing enzymes (white colonies on MacConkey agar containing D-galactose). In the absence of L-arabinose, cells can utilize D-galactose (red colonies).
    Figure Legend Snippet: ( A ) Chromosomal construction of Escherichia coli HK1160 ( P BAD -dCas9-NG-KmR) and structure of sgRNA plasmid. dCas9-NG protein is expressed by L-arabinose-inducible P BAD promoter. sgRNA is constitutively expressed by P j23119 promoter. ( B ) Regulation of gal operon transcription by dCas9-NG and sgRNA complex. In the absence of L-arabinose, dCas9-NG is not expressed and gal transcription can be initiated by RNA polymerase. In the presence of L-arabinose, dCas9-NG is expressed and dCas9-NG-sgRNA complex recognizes target DNA adjacent to functional PAM sequence. ( C ) Switch on/off of L-arabinose-inducible CRISPRi. In the presence of L-arabinose, Cas9 and dCas9 are expressed to repress the expression of D-galactose metabolizing enzymes (white colonies on MacConkey agar containing D-galactose). In the absence of L-arabinose, cells can utilize D-galactose (red colonies).

    Techniques Used: Plasmid Preparation, Functional Assay, Sequencing, Expressing



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    cas9 ng gene  (Integrated DNA Technologies)
    99
    Integrated DNA Technologies cas9 ng gene
    ( A ) Chromosomal construction of Escherichia coli HK1160 ( P BAD -dCas9-NG-KmR) and structure of sgRNA plasmid. dCas9-NG protein is expressed by L-arabinose-inducible P BAD promoter. sgRNA is constitutively expressed by P j23119 promoter. ( B ) Regulation of gal operon transcription by dCas9-NG and sgRNA complex. In the absence of L-arabinose, dCas9-NG is not expressed and gal transcription can be initiated by RNA polymerase. In the presence of L-arabinose, dCas9-NG is expressed and dCas9-NG-sgRNA complex recognizes target DNA adjacent to functional PAM sequence. ( C ) Switch on/off of L-arabinose-inducible CRISPRi. In the presence of L-arabinose, <t>Cas9</t> and dCas9 are expressed to repress the expression of D-galactose metabolizing enzymes (white colonies on MacConkey agar containing D-galactose). In the absence of L-arabinose, cells can utilize D-galactose (red colonies).
    Cas9 Ng Gene, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9+ng+gene/pmc09728369-69-5-14?v=Integrated+DNA+Technologies
    Average 99 stars, based on 1 article reviews
    cas9 ng gene - by Bioz Stars, 2026-07
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Chromosomal construction of Escherichia coli HK1160 ( P BAD -dCas9-NG-KmR) and structure of sgRNA plasmid. dCas9-NG protein is expressed by L-arabinose-inducible P BAD promoter. sgRNA is constitutively expressed by P j23119 promoter. ( B ) Regulation of gal operon transcription by dCas9-NG and sgRNA complex. In the absence of L-arabinose, dCas9-NG is not expressed and gal transcription can be initiated by RNA polymerase. In the presence of L-arabinose, dCas9-NG is expressed and dCas9-NG-sgRNA complex recognizes target DNA adjacent to functional PAM sequence. ( C ) Switch on/off of L-arabinose-inducible CRISPRi. In the presence of L-arabinose, Cas9 and dCas9 are expressed to repress the expression of D-galactose metabolizing enzymes (white colonies on MacConkey agar containing D-galactose). In the absence of L-arabinose, cells can utilize D-galactose (red colonies).

    Journal: Journal of Microbiology and Biotechnology

    Article Title: Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference

    doi: 10.4014/jmb.2008.08058

    Figure Lengend Snippet: ( A ) Chromosomal construction of Escherichia coli HK1160 ( P BAD -dCas9-NG-KmR) and structure of sgRNA plasmid. dCas9-NG protein is expressed by L-arabinose-inducible P BAD promoter. sgRNA is constitutively expressed by P j23119 promoter. ( B ) Regulation of gal operon transcription by dCas9-NG and sgRNA complex. In the absence of L-arabinose, dCas9-NG is not expressed and gal transcription can be initiated by RNA polymerase. In the presence of L-arabinose, dCas9-NG is expressed and dCas9-NG-sgRNA complex recognizes target DNA adjacent to functional PAM sequence. ( C ) Switch on/off of L-arabinose-inducible CRISPRi. In the presence of L-arabinose, Cas9 and dCas9 are expressed to repress the expression of D-galactose metabolizing enzymes (white colonies on MacConkey agar containing D-galactose). In the absence of L-arabinose, cells can utilize D-galactose (red colonies).

    Article Snippet: Second, 3’-part (837 bp) of cas9-NG gene was codon-optimized using Codon Optimization Tool ( https://sg.idtdna.com/pages/tools/codon-optimization-tool ) and chemically synthesized (Integrated DNA Technologies, USA).

    Techniques: Plasmid Preparation, Functional Assay, Sequencing, Expressing